ata-100 advanced tissue arrayer Search Results


fresh lb  (ATCC)
99
ATCC fresh lb
Fresh Lb, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/Acinetobacter+baumannii+Bouvet+and+Grimont/pmc03416510-121-12-14
Average 99 stars, based on 1 article reviews
fresh lb - by Bioz Stars, 2026-10
99/100 stars
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short interfering (si)rnas targeting lin7c  (Ribobio co)
90
Ribobio co short interfering (si)rnas targeting lin7c
Short Interfering (Si)rnas Targeting Lin7c, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/short+interfering++si+rnas+targeting+lin7c/pm35761801-49-9-65
Average 90 stars, based on 1 article reviews
short interfering (si)rnas targeting lin7c - by Bioz Stars, 2026-10
90/100 stars
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p350 ata-amab91164-100  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH p350 ata-amab91164-100
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
P350 Ata Amab91164 100, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/p350+ata+amab91164+100/pmc09561781-332-37-40
Average 90 stars, based on 1 article reviews
p350 ata-amab91164-100 - by Bioz Stars, 2026-10
90/100 stars
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rabbit polyclonal anti atm antibody  (Proteintech)
95
Proteintech rabbit polyclonal anti atm antibody
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Rabbit Polyclonal Anti Atm Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/ATM+Antibody/pm30657058-191-8-6
Average 95 stars, based on 1 article reviews
rabbit polyclonal anti atm antibody - by Bioz Stars, 2026-10
95/100 stars
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80 gb ultra ata/100 hard drive  (apple inc)
90
apple inc 80 gb ultra ata/100 hard drive
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
80 Gb Ultra Ata/100 Hard Drive, supplied by apple inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/80+gb+ultra+ata+100+hard+drive/pmc02847392-29-31-12
Average 90 stars, based on 1 article reviews
80 gb ultra ata/100 hard drive - by Bioz Stars, 2026-10
90/100 stars
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si-akt3  (Ribobio co)
90
Ribobio co si-akt3
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Si Akt3, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/akt3+sirna/pm30535467-57-39-48
Average 90 stars, based on 1 article reviews
si-akt3 - by Bioz Stars, 2026-10
90/100 stars
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anti phospho atm  (Rockland Immunochemicals)
93
Rockland Immunochemicals anti phospho atm
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Anti Phospho Atm, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/ATM+phospho+S1981+Biotin+Conjugated+Antibody/pmc03010519-95-30-32
Average 93 stars, based on 1 article reviews
anti phospho atm - by Bioz Stars, 2026-10
93/100 stars
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gold (100 nm) sensor crystals (qsx301)  (ATA Scientific)
90
ATA Scientific gold (100 nm) sensor crystals (qsx301)
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Gold (100 Nm) Sensor Crystals (Qsx301), supplied by ATA Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/gold+coated+qcm+crystals/us12044651-826-0-9
Average 90 stars, based on 1 article reviews
gold (100 nm) sensor crystals (qsx301) - by Bioz Stars, 2026-10
90/100 stars
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disposable polycarbonate cuvette dts1061  (ATA Scientific)
90
ATA Scientific disposable polycarbonate cuvette dts1061
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Disposable Polycarbonate Cuvette Dts1061, supplied by ATA Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/disposable+polycarbonate+cuvette+dts1061/pm37016733-89-20-24
Average 90 stars, based on 1 article reviews
disposable polycarbonate cuvette dts1061 - by Bioz Stars, 2026-10
90/100 stars
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rabbit anti cpe  (Atlas Antibodies)
92
Atlas Antibodies rabbit anti cpe
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Rabbit Anti Cpe, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/Anti-CPE/pmc08226255-73-123-127
Average 92 stars, based on 1 article reviews
rabbit anti cpe - by Bioz Stars, 2026-10
92/100 stars
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dithiothreitol  (Thermo Fisher)
99
Thermo Fisher dithiothreitol
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Dithiothreitol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/Triton+X-100/10__1128_slash_mbio__01841___19-308-23-44
Average 99 stars, based on 1 article reviews
dithiothreitol - by Bioz Stars, 2026-10
99/100 stars
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chamber hbo exposure  (Nitrox LLC)
90
Nitrox LLC chamber hbo exposure
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Chamber Hbo Exposure, supplied by Nitrox LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ata-100+advanced+tissue+arrayer/chamber+hbo+exposure/pm37207635-180-26-49
Average 90 stars, based on 1 article reviews
chamber hbo exposure - by Bioz Stars, 2026-10
90/100 stars
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Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes (p350) ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.

Journal: Advanced Science

Article Title: Trip13 Depletion in Liver Cancer Induces a Lipogenic Response Contributing to Plin2‐Dependent Mitotic Cell Death

doi: 10.1002/advs.202104291

Figure Lengend Snippet: Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes (p350) ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.

Article Snippet: Plin1 (ab61682), Plin2 (ab108323 and ab52356), Plin3 (ab47638), Plin4 (ab234752), Plin5 (ab222811), sterol regulatory‐element binding protein (SREBP) (ab28481), Mad2 (ab97777), GFP (ab290), pericentrin (ab4448), CD36 (ab133625), tubulin (ab7291), and valosin‐containing protein (VCP) (ab11433) were ordered from abcam, P350 (ATA‐AMAB91164‐100) from Biozol, Trip13 (19602‐1‐AP) from Proteintech, and INSR (sc‐711), FASN (sc‐20140) from SantaCruz Biotech.

Techniques: Control, Transfection, Western Blot, Targeted Proteomics, Comparison